Effect of ethyl methane sulphonate on callus growth of clusterbean [Cyamopsis tetragonoloba (L.) Taub.]
Deepika, H. R. Dhingra, Minakshi Pal, S. K. Pahuja and Priyanka
Department of Botany & Plant Physiology
CCS Haryana Agricultural University,
Hisar-125 004 (Haryana), India
(Received : 2 November 2015; Accepted : 15 December 2015)
SUMMARY
The present study was carried out to study the effect on ethyl methane sulphonate on callus growth of clusterbean. . The experiment was put in process at department of botany and
Plant Physiology in 2011. Seeds of clusterbean HG 2-20 were injucted with the various doses
of EMS (0.5%, 0.75% and 1.0%) for different duration of time (6 hr and 8 hr). The mutated
seed were cultured on germination media for obtaining explants i.e. cotyledonary node. It was
then grown on callusing media. Fresh and dry weight of the callus was recorded recorded after
one month. From results, it was found that 0.75 per cent EMS concentration was more effective in callus growth, while the 1 per cent EMS was found to the lethal dose.
Key words:Callus, Cyamopsis tetragonoloba, EMS, guar,