GENETIC DIVERSITY IN F2 SEGREGATING POPULATION OF SINGLECUT AND MULTI-CUT VARIETY OF SORGHUM
ANITA YADAV, PARDEEP KUMAR*, K. S. BOORA AND S. K. PAHUJA
Department of Molecular Biology, Biotechnology & Bioinformatics
CCS Haryana Agricultural University,
Hisar-125 004 (Haryana), India
*(e-mail : pardeep1@icar.gov.in)
(Received : 15 January 2017; Accepted : 20 March 2017)

SUMMARY

The F2 segregating population of sorghum [Sorghum bicolor (L.) Moench] of cross HJ 541 (singlecut) × SSG 59-3 (multi-cut) was studied by using simple sequence repeats (SSR) markers to measure genetic divergence within the population. Out of 50 SSR markers, 21 were found to be polymorphic which showed 90.3 per cent polymorphism. Cluster tree analysis grouped the 32 F2 progeny lines into two major clusters : Cluster I comprised three F2 progeny lines with parental genotype, HJ 541, whereas cluster II had the remaining 29 F2 progeny lines with parental genotype, SSG 59-3. Genetic similarity among F2 progeny lines ranged from 0.45 to 0.81 with average similarity of 0.66. Parental genotypes of sorghum were found to be most diverse and F2 progeny lines viz., G2, G18, G20, G21 and G29 showed high similarity with multi-cut parental sorghum genotype, SSG 59-3. Hence, these F2 progeny lines could be selected for early variety development from the F2 segregating population in order to meet ever increasing demand of green fodder for the livestock.

Key words: Genetic diversity, SSR markers, Single-cut sorghum, multi-cut sorghum, segregating population

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