ALLELE SPECIFIC DIVERSITY USING FERTILITY RESTORATION
RELATED SSR MARKERS IN SORGHUM [SORGHUM BICOLOR (L.) MOENCH]
P. CHAUHAN*, P. K. PANDEY, P. K. SHROTRIA, N. K. SINGH, G. PANDEY AND S. SINGH
Department of Genetics & Plant Breeding
G. B. Pant University of Agriculture & Technoloy,
Pantnagar-263 145 (Uttarakhand), India
*(e-mail : priyam09chauhan@gmail.com)
(Received : 05 January 2015; Accepted : 28 February 2015)
SUMMARY
A total of five SSR markers were used for the estimation of allelic diversity for fertility restorer gene in 17 sorghum genotypes consisting of five male sterile lines (female), four maintainer lines and eight pollinators (male) lines. The experiment was conducted in UG Plant Biotechnology Lab of the
Department of Genetics & Plant Breeding, G. B. Pant University of Agriculture & Technology, Pantnagar (U. S. Nagar). All markers were found to be polymorphic for all sorghum genotypes. Polymorphism ranged from 50% (Xtxp250) to 100 per cent (Drenshsbm 95). Among all 22 alleles, the number of alleles per locus varied from minimum of 2 to maximum of 7, with average alleles of 4.4 per locus. The PIC for all five primers varied from 0.50 (Drenshsbm 95) to 0.87 (TS304T), with a mean of 0.73. The genetic similarity (GS) varied from 0.55 to 1.0 for all genotypes. Among all three clusters, cluster 3 was found to be most diverse having 0.472 allelic diversity for rf1 gene. Dendrogram revealed that male sterile lines ICSA 467 and ICSA 276 showed more genetic diversity with restorers CS3541, PANTCHARI 5 and CSV 15 and these combinations can be used as heterotic parents in improvement breeding programme of sorghum.
Key words: Sorghum bicolor, SSRs, allelic diversity, fertility restorer gene