MORPHOLOGICAL VARIATIONS INDUCED BY ETHYL METHANE SULPHONATE IN CLUSTERBEAN [CYAMOPSIS TETRAGONOLOBA (L.) TAUB.]
DEEPIKA*, MINAKSHI PAL AND S. K. PAHUJA
Department of Botany & Plant Physiology
CCS Haryana Agricultural University,
Hisar-125 004 (Haryana), India
*(e-mail : deepikahau84@gmail.com)
(Received : 02 November 2016; Accepted : 08 March 2016)
SUMMARY
The present investigation was carried out to observe the different morphological variations induced
by chemical mutagen ethyl methane sulphonate (EMS) on clusterbean commonly known as guar. Guar is an important forage crop with versatile properties. The importance of guar is mainly due to presence of guar gum produced from its seeds. EMS is a chemical potent mutagen, capable of inducing large number of mutations. Various morphological variations were produced due to treatment of EMS. Seed treated with 0.5 per cent EMS for 6 h produced more variable results as compared to 0.5 per cent, 8 h treatment. Incubation of seed in 1 per cent EMS for both 6 and 8 h was inhibitory, except to one major variation, which produced longer root and shoot as compared to the untreated seed. 1 per cent EMS resulted in more inhibitory/toxic effect on germination of guar. The concentration of 1 per cent EMS was not found to be suitable for inducing mutagenic effects.
Key words:Cyamopsis tetragonoloba, guar, EMS, morphological variations