MOLECULAR CHARACTERIZATION AND GENETIC DIVERGENCE
REVEALED BY RAPD IN OATS (AVENA SATIVA L.)
YOGESH RUWALI* AND INDRA DEO
Department of Genetics & Plant Breeding
G. B. Pant University of Agriculture & Technology,
Pantnagar-263 145 (Uttarakhand), India
*(e-mail : ruwali.yogesh@gmail.com)
(Received : 18 December 2014; Accepted : 29 January 2015)
SUMMARY
Genetic divergence among oat genotypes from three different geographical blocks constituting nine
oat genotypes viz., KENT, WRIGHT, OS-6, JHO-822, OL-9, JHO-851, OL-88, UPO-94 and UPO-212 was analyzed by Random Amplified Polymorphic DNA method in Department of Genetics & Plant Breeding, College of Agriculture, G. B. P. U. A. & T., Pantnagar. The results showed that the diversity among oat varieties was high at DNA level. Nine primers selected from 15 RAPD primers could amplify 245 clear and identifiable bands, of which 236 bands were polymorphic, accounting for 96.32 per cent genetic polymorphism. All oat genotypes studied could be distinctly divided into two major groups with the genetic distance level at 0.60 by cluster analysis based on the Jaccard’s coefficient of similarity. The cluster break was further supported by the pedigree relationships among the genotypes. Several unique identifiable bands were also found in different genotypes which would help in characterization of these genotypes. The results found were encouraging and
advocated that RAPD technique could be used for classification, identification and evolution studies.
Key words: Oats, divergence, RAPD, cluster