GENOTYPIC DIVERSITY IN PEARL MILLET [PENNISETUM GLAUCUM (L.) R. BR.] MAINTAINER LINES USING SSR MARKERS
JYOTI KAUSHIK*, DEV VART, MAMTA NEHRA AND MUKESH KUMAR
Department of Genetics and Plant Breeding
CCS Haryana Agricultural University, Hisar- 125004 (Haryana), India
*(e-mail : firstname.lastname@example.org)
(Received : 23 July 2019; Accepted :20 September 2019)
Pearl Millet [Pennisetum glaucum (L.) R.Br.] is one of the staple food and fodder crop of the arid and semi-arid regions of Africa and Asia. Pearl millet displays enormous phenotypic and genotypic diversity. Assessment of available genetic diversity in pearl millet through the use of molecular markers will supplement the conventional breeding programme. Keeping this in view, the present study was undertaken to evaluate genetic diversity among forty eight maintainer lines of pearl millet with twenty two SSR primers which produced a total of 85 alleles. Three out of twenty two polymorphic primers showed PIC value of more than 0.70 i.e. Xpsmp2066, Xpsmp2089 and Xpsmp2063. The maximum number of ten amplified products was observed in the profiles of the primer Xpsmp2001. The highest PIC value is observed for Xpsmp2089 with value (0.78). The molecular data grouped the forty eight genotypes of pearl millet into eight main clusters which revealed considerable genetic diversity among the maintainer lines. Among the eight clusters, cluster II was the largest comprising eighteen genotypes. So, SSRs are effective markers for the assessment of genetic diversity in maintainer lines of pearl millet. The diversity assessed can be manipulated to broaden the genetic base of maintainer line for the development of commercial hybrid varieties.
Key words:Pearl millet, genotypic diversity, SSR primers, molecular data, clustering