ANSHU BAJAJ AND SURENDER K. PAHUJA
Department of Biotechnology and Molecular Biology,
CCS Haryana Agricultural University, Hisar-125 004 (Haryana), India
Department of Plant Breeding, CCS Haryana Agricultural University, Hisar-125 004 (Haryana), India
*(e-mail: pahujask66@gmail.com)
(Received: 20 September 2024; Accepted: 3 October 2024)
SUMMARY
Guar is a legume crop resistant to drought and susceptible to diseases like bacterial blight and alternaria leaf spot. Its a major crop in various developed and developing countries. It has been producing guar gum for industrial uses. In the present investigation bacterial blight resistant QTL were identified using RAPD markers. Statistical analysis was done using Mapmanager QTX software. DNA estimation was done on uv spectrophotometer, further analysed by gel electrophoresis for quality. Resistant and susceptible genotypes were used to generate F2-3 population used further for linkage map construction for the disease bacterial blight. F2-3 population was undertaken for disease percent survival in contrast to molecular marker analysis for the disease. Polymorphism studies were undertaken among molecular markers, molecular mapping was done and distances were calculated at p=0.05 markers linked to each other for disease were selected Molecular mapping was done with three markers covering a map distance of 43.9 cM. Two markers (D1 &AB7) were found linked to each other and were grouped together in group 2 whereas L19 was found unlinked to other markers and contributing to disease resistance. These QTLs can be used for creation of superior genotypes of clusterbean using molecular breeding techniques for resistance to bacterial blight.
Key words: Bacterial blight, QTLs, Molecular mapping, Clusterbean, Polymorphism